《植物生理学报》 2010, 46(1): 47-50

盐穗木甜菜碱醛脱氢酶基因(BADH)的克隆及其在盐胁迫下的表达分析

关波, 胡有贞, 张富春, 曾幼玲*

新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐 830046

通信作者：曾幼玲；E-mail: zengyou ling@xju.edu.cn；Tel: 0991- 85 832 59

摘　要：

根据我们实验室已发表的植物甜菜碱醛脱氢酶基因(BADH)的同源保守区设计引物, 通过RT-PCR扩增获得了由1 503 个核苷酸组成的盐穗木BADH 基因开放阅读框, 推测该基因编码500个氨基酸, 分子量约为54.49 kDa的多肽。推测的盐穗 木 BADH 氨基酸序列中包含一段甜菜碱醛脱氢酶中高度保守的十肽序列(VTLEL GGKSP)以及与酶功能有关的半胱氨酸 残基(Cys)。序列比对结果显示, 盐穗木 BADH 与盐地碱蓬、中亚滨藜、盐爪爪以及菠菜等的核苷酸序列同源性在 81% 以 上, 与水稻的同源性也达到 68%。半定量 RT-PCR 分析结果表明, 盐穗木 BADH 基因的表达受盐胁迫诱导, 推测 BADH 可 能与盐穗木具有较强的耐盐能力有关。

关键词：盐穗木; 甜菜碱醛脱氢酶; 基因克隆; 表达分析

收稿：2009-10-22   修定：2010-01-04

资助：校院联合资助项目(070196)和新疆维吾尔自治区科技重大专项(200731138-3)。

Molecular Cloning and Expression Analysis of Betaine-Aldehyde Dehydrogenase Gene from Halostachys caspica (Bieb.) C. A. Mey

GUAN Bo, HU You-Zhen, ZHANG Fu-Chun, ZENG You-Ling*

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

Corresponding author: ZENG You-Ling; E-mail: zengyou ling@xju.edu.cn; Tel: 0991- 85 832 59

Abstract:

The complete open reading frame of betaine-aldehyde dehydrogenase (BADH) gene was cloned from Halostachys caspica using the method of reverse transcription PCR (RT-PCR), and the primers were designed according to the published sequences of BADH cDNA. The sequence of BADH was 1 503 bp, encoding a 54.49 kDa protein of 500 amino acids. The deduced amino acid sequence contained a conserved decapeptide sequence (VTLEL GGKSP) of BADH and cysteine associated with enzyme function. The results of sequence alignment showed that HcBADH shared 81% and above homology with BADHs from Suaeda salsa, Atriplex centralasiatica, Kalidium foliatum and Spinacia oleracea, and shared a 68% homology with Oryza sativa. The results of semiquantitative RT-PCR revealed that the expression of HcBADH gene was induced by salt stress, and BADH might be related to the salt tolerance of Halostachys caspica.

Key words: Halostachys caspica; betaine-aldehyde dehydrogenase; gene cloning; expression analysis